Biodiversity and efficacy of fungal isolates associated with Kenyan populations of potato cyst nematode (Globodera spp.)

Abstract

Potato Cyst Nematodes (PCN) (Globodera rostochiensis and G. pallida Woll.) are quarantine pests of potato (Solanum tuberosum L.) worldwide capable of causing significant yield loss and difficult to manage with conventional methods. The study explored the diversity of antagonistic fungi associated with PCN obtained from soil samples collected in Nyandarua and Nakuru Counties in Kenya and their effect on PCN egg viability and hatching was also evaluated. Twelve fungal isolates from five genera were isolated and characterized using morphological and molecular techniques. The twelve isolates were Trichoderma asperellum (4), T. hamatum, T. breve, T. atrobruneum, Amanita basiorubra, Setophoma terrestris (2), Penicillium chrysogenum and Clonostachys rosea. The most abundant isolate was Trichoderma spp. with 58% occurrence. The effect of seven of the isolates on PCN eggs showed that T. breve and P. chrysogenum reduced egg viability by 41% and 34%, respectively while T. asperellum and T. breve reduced their hatching by 50% on average. Trichoderma atrobrunneum, T. hamatum, and A. basiorubra also reduced the PCN egg viability by 27% on average. These fungal isolates could provide a potential tool for PCN management in potato production systems for improved yields. However, further studies are warranted to validate these findings under greenhouse and field conditions. A more comprehensive bioprospecting survey for PCN associated antagonistic fungi needs to be extended to other potato growing regions to explore further cyst pathogens.