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Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya.

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dc.contributor.author Gichuhi, J.
dc.contributor.author Khamis, F.
dc.contributor.author Van den Berg, J.
dc.contributor.author du Plessis, H.
dc.contributor.author Ekesi, Sunday.
dc.contributor.author Herren, J.
dc.date.accessioned 2021-06-10T11:43:10Z
dc.date.available 2021-06-10T11:43:10Z
dc.date.issued 2020
dc.identifier.uri http://hdl.handle.net/123456789/1473
dc.description Research Article en_US
dc.description.abstract Background The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. frugiperda could play a role in the insects’ success and adaptability. However, bacterial communities in S. frugiperda remain poorly studied. Methods We investigated the composition, abundance and diversity of microbiomes associated with larval and adult specimens of S. frugiperda collected from four maize growing regions in Kenya through high throughput sequencing of the bacterial 16S rRNA gene. The population structure of S. frugiperda in Kenya was assessed through amplification of the mitochondrial cytochrome oxidase subunit I gene. Results We identified Proteobacteria and Firmicutes as the most dominant bacterial phyla and lesser proportions of Bacteroidetes and Actinobacteria. We also observed differences in bacterial microbiome diversity between larvae and adults that are a likely indication that some prominent larval bacterial groups are lost during metamorphosis. However, several bacterial groups were found in both adults and larvae suggesting that they are transmitted across developmental stages. Reads corresponding to several known entomopathogenic bacterial clades as well as the fungal entomopathogen, Metarhizium rileyi, were observed. Mitochondrial DNA haplotyping of the S. frugiperda population in Kenya indicated the presence of both “Rice” and “Corn” strains, with a higher prevalence of the “Rice” strain. en_US
dc.description.sponsorship European Union Funded project “Integrated pest management” strategy to counter the 283 threat of invasive fall armyworm to food security in Eastern Africa (FAW‐IPM) (FOOD/2018/402‐634); UK 284 Aid from the UK Government; Swedish International Development Cooperation Agency (Sida); the Swiss 285 Agency for Development and Cooperation (SDC); and the Kenyan Government. en_US
dc.publisher PeerJ en_US
dc.rights Attribution-NonCommercial-ShareAlike 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/us/ *
dc.subject Fall armyworm en_US
dc.subject Spodoptera frugiperda en_US
dc.subject gut bacteria en_US
dc.subject 16S sequencing en_US
dc.subject corn strain en_US
dc.subject rice strain en_US
dc.subject mtDNA haplotype en_US
dc.title Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya. en_US
dc.type Article en_US


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