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Unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens

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dc.contributor.author Vincent, Doublet
dc.contributor.author Yvonne, Poeschl
dc.contributor.author Andreas, Gogol-Döring
dc.contributor.author Cédric, Alaux
dc.contributor.author Desiderato, Annoscia
dc.contributor.author Christian, Aurori
dc.contributor.author Seth, M. Barribeau
dc.contributor.author Oscar, C. Bedoya-Reina
dc.contributor.author Mark, J. F. Brown
dc.contributor.author James, C. Bull
dc.contributor.author Michelle, L. Flenniken
dc.contributor.author David, A. Galbraith
dc.contributor.author Elke, Genersch
dc.contributor.author Sebastian, Gisder
dc.contributor.author Ivo, Grosse
dc.contributor.author Holly, L. Holt
dc.contributor.author Dan, Hultmark
dc.contributor.author H. Michael, G. Lattorff
dc.contributor.author Yves, Le Conte
dc.contributor.author Fabio, Manfredini
dc.contributor.author Dino, P. McMahon
dc.contributor.author Robin, F. A. Moritz
dc.contributor.author Francesco, Nazzi
dc.contributor.author Elina, L. Niño
dc.contributor.author Katja Nowick
dc.contributor.author Ronald, P. van Rij
dc.contributor.author Robert, J. Paxton
dc.contributor.author Christina, M. Grozinger
dc.date.accessioned 2021-06-07T08:33:59Z
dc.date.available 2021-06-07T08:33:59Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/123456789/1396
dc.description.abstract Background: Organisms typically face infection by diverse pathogens, and hosts are thought to have developed specific responses to each type of pathogen they encounter. The advent of transcriptomics now makes it possible to test this hypothesis and compare host gene expression responses to multiple pathogens at a genome-wide scale. Here, we performed a meta-analysis of multiple published and new transcriptomes using a newly developed bioinformatics approach that filters genes based on their expression profile across datasets. Thereby, we identified common and unique molecular responses of a model host species, the honey bee (Apis mellifera), to its major pathogens and parasites: the Microsporidia Nosema apis and Nosema ceranae, RNA viruses, and the ectoparasitic mite Varroa destructor, which transmits viruses. Results: We identified a common suite of genes and conserved molecular pathways that respond to all investigated pathogens, a result that suggests a commonality in response mechanisms to diverse pathogens. We found that genes differentially expressed after infection exhibit a higher evolutionary rate than non-differentially expressed genes. Using our new ioinformatics approach, we unveiled additional pathogen-specific responses of honey bees; we found that apoptosis appeared to be an important response following microsporidian infection,while genes from the immune signalling pathways, Toll and Imd, were differentially expressed after Varroa/virus infection. Finally, we applied our bioinformatics approach and generated a gene co-expression network to identifyhighly connected (hub) genes that may represent important mediators and regulators of anti-pathogen responses.Conclusions: Our meta-analysis generated a comprehensive overview of the host metabolic and other biological . processes that mediate interactions between insects and their pathogens. We identified key host genes and pathways that respond to phylogenetically diverse pathogens, representing an important source for future functional studies as well as offering new routes to identify or generate pathogen resilient honey bee stocks. The statistical and bioinformatics approaches that were developed for this study are broadly applicable to synthesize information across transcriptomic datasets. These approaches will likely have utility in addressing a variety of biological questions en_US
dc.description.sponsorship TRANSBEE sDiv The Synthesis Centre for Biodiversity Sciences within the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig The German Science Foundation (FZT 118) Insect Pollinators Initiative UK-USA exchange funded by the BBSRC The IPI is funded jointly by the Biotechnology and Biological Sciences Research Council, the Department for Environment, Food and Rural Affairs, the Natural Environment Research Council, the Scottish Government Wellcome Trust en_US
dc.publisher BMC Genomic en_US
dc.rights Attribution-NonCommercial-ShareAlike 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/us/ *
dc.subject Apis mellifera en_US
dc.subject Nosema en_US
dc.subject Varroa destructor en_US
dc.subject DWV en_US
dc.subject IAPV en_US
dc.subject RNA virus en_US
dc.subject Meta-analysis en_US
dc.subject Transcriptomics en_US
dc.subject Coexpression network en_US
dc.subject Immunity en_US
dc.title Unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens en_US
dc.type Article en_US


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