Bio-Prospecting for Insecticidal Scorpion Venoms and Identification of Bioactive Toxins

Ochola, John Bwire

dc.contributor.author	Ochola, John Bwire
dc.date.accessioned	2019-10-03T09:30:04Z
dc.date.available	2019-10-03T09:30:04Z
dc.date.issued	2005
dc.identifier.uri	http://hdl.handle.net/123456789/1007
dc.description	A Thesis Submitted in Partial Fulfillment for the Degree of Master of Science in Chemistry in the Jomo K.enyatta University of Agriculture and Technology	en_US
dc.description.abstract	Scorpion venom contains insect and mammal selective toxins. However, despite their significance and potential application in insect-pest control, bioactivity of Kenyan scorpions is still unknown. As such this study initiated investigations on the inherent insecticidal activity of the venoms of scorpions collected in Kenya. Venom from four scorpion species namely Parabuthus leiosoma, Parabuthus pallidus, Hottentotta trilimeatus and Hottentotta eminii was collected upon stinging a parafilm ·membrane. Crude venom was extracted by homogenizatio and centrifugation then the supernatant was lyophilized. Bioassay guided purification was carried out by cation exchange chromatography and reverses-phase chromatography. Amino acid sequence of the active fractions was determined by N-terminal automated Edman degradation. The structure elucidation of pure fractions was confirmed using LC-MS and UV. ...(continue from the attached PDF)	en_US
dc.description.sponsorship	Dissertation Research Internship Programme (DRIP).	en_US
dc.publisher	Jomo K.enyatta University of Agriculture and Technology	en_US
dc.rights	Attribution-NonCommercial-ShareAlike 3.0 United States	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/3.0/us/	*
dc.subject	Parabuthus pallidus	en_US
dc.subject	Parabuthus leiosoma,	en_US
dc.subject	Hottentotta trilimeatus	en_US
dc.subject	Hottentotta eminii	en_US
dc.title	Bio-Prospecting for Insecticidal Scorpion Venoms and Identification of Bioactive Toxins	en_US
dc.type	Thesis	en_US